Pseudomonas Aeruginosa Water Analysis

Pseudomonas aeruginosa is an important water-borne pathogen that widely exists in various types of water and has strong resistance to physical and chemical factors such as disinfectants, drying, ultraviolet rays, and adverse environments. The bacteria can cause wounds, respiratory and urinary tract infections, and even sepsis. At the same time, a variety of pathogenic factors produced by it are likely to cause purulent infection and drug resistance in immunocompromised and suppressed people or people who have used antibiotics for a long time. Therefore, how quickly and accurately identifying Pseudomonas aeruginosa is of great significance for ensuring water source and human safety.

BOC Sciences offers a comprehensive Pseudomonas aeruginosa water testing program to assess the risk to your facility followed by everything you need. Our methods are suitable for the analysis of drinking water as well as water from swimming pools, taps, dialysis and well water, among others. By steadfastly following the facts and maintaining the highest scientific standards, our results will consistently rank among the best in the industry.

Fig. 1 Schematic representation of main infections caused by Pseudomonas aeruginosa.^[1]

Detection Technologies

Early detection is critical to treating Pseudomonas aeruginosa infection. Our sole motivation has long been to help you develop rapid and sensitive assays for Pseudomonas aeruginosa. According to the specific situation of the water sample, we formulate a reasonable detection plan to ensure the high accuracy of the test results.

Conventional bacterial culture methods

One of our methods is to complete the laboratory detection of Pseudomonas aeruginosa based on a fully automatic microbial biochemical identification instrument. Automated systems have many advantages, such as a high degree of automation, simple operating procedures, improved sample handling, good reproducibility and accuracy, etc. Notably, our automated system not only recognizes Pseudomonas aeruginosa, but also performs Antimicrobial Susceptibility Testing (AST).

Real-time, fluorescence-based quantitative PCR

By introducing fluorescent molecules into the reaction mixture, the PCR reaction can be monitored in real-time to achieve quantitative detection. This method enables the detection of Pseudomonas aeruginosa in a relatively short time and provides definitive results without additional confirmation steps.

MALDI-TOF MS assays

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a new type of soft ionization mass spectrometry used to map proteins in microorganisms. It compares the mass spectrometry data of clinical microorganisms with the standard protein database of known microorganisms to achieve the purpose of identification. Due to its rapidity, accuracy, sensitivity, automation, and high throughput, we have applied this technique to identify Pseudomonas aeruginosa in water environments.

Precautions for sample collection

• Meet minimum sample size requirements.
• Water samples must be collected on the day of collection (this is critical for accurate analysis).
• To analyze more than one sample, it is necessary to collect all samples at the same time.
• Strictly follow the delivery requirements (heat insulation packaging, ice packs, etc.) when sending samples.
• Make sure to use one vial per sample.

If you would like to do regular testing, please contact us for standing orders. We can provide you with a custom quote. BOC Sciences solutions are trusted by many customers.